Affiliation:
1. Department of Microbiology and Molecular Genetics
2. Computational Genomic Section, Human Genetics Center, The University of Texas Health Science Center, Houston, Texas 77030
Abstract
ABSTRACT
Three strains of
Rhodobacter sphaeroides
of diverse origin have been under investigation in our laboratory for their genome complexities, including the presence of multiple chromosomes and the distribution of essential genes within their genomes. The genome of
R. sphaeroides
2.4.1 has been completely sequenced and fully annotated, and now two additional strains (ATCC 17019 and ATCC 17025) of
R. sphaeroides
have been sequenced. Thus, genome comparisons have become a useful approach in determining the evolutionary relationships among different strains of
R. sphaeroides
. In this study, the concatenated chromosomal sequences from the three strains of
R. sphaeroides
were aligned, using Mauve, to examine the extent of shared DNA regions and the degree of relatedness among their chromosome-specific DNA sequences. In addition, the exact intra- and interchromosomal DNA duplications were analyzed using Mummer. Genome analyses employing these two independent approaches revealed that strain ATCC 17025 diverged considerably from the other two strains, 2.4.1 and ATCC 17029, and that the two latter strains are more closely related to one another. Results further demonstrated that chromosome II (CII)-specific DNA sequences of
R. sphaeroides
have rapidly evolved, while CI-specific DNA sequences have remained highly conserved. Aside from the size variation of CII of
R. sphaeroides
, variation in sequence lengths of the CII-shared DNA regions and their high sequence divergence among strains of
R. sphaeroides
suggest the involvement of CII in the evolution of strain-specific genomic rearrangements, perhaps requiring strains to adapt in specialized niches.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
62 articles.
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