Rapid Differentiation Between Nocardia and Streptomyces by Paper Chromatography of Whole-Cell Hydrolysates

Author:

Becker B.1,Lechevalier Mary P.1,Gordon Ruth E.1,Lechevalier H. A.1

Affiliation:

1. Institute of Microbiology, Rutgers, The State University, New Brunswick, New Jersey

Abstract

Whole-cell hydrolysates were prepared from 58 strains of nocardiae and streptomycetes. Strains morphologically intermediate between the two genera and morphological variants of the same strains were included. Paper chromatograms made from the whole-cell hydrolysates clearly demonstrated meso -diaminopimelic acid as a major constituent of cultures of Nocardia spp., and LL-diaminopimelic acid as a major constituent of cultures of Streptomyces spp. In cultures of ten strains of N. madurae and of three of N. pelletieri, meso -diaminopimelic acid predominated, thereby supporting the assignment of these species to the genus Nocardia .

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference12 articles.

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2. La composition chimique des parois cellulaires d'actinomyc 6tes et son application taxonomique;CUMMINS C. S.;Ann. Inst. Pasteur,1962

3. Chemical composition and antigenic structure of cell walls of Coryrebacteri um, Mycobacterium, Nocardia, Actinomyces and Arthrobacter;CUMMINS C. S.;J. Gen. Microbiol.,1962

4. Studies on the cell-wall composition and taxonomy of Actinomycetales and related groups. J;CUMMINS C. S.;Gen. Microbiol.,1958

5. The type species of the genus Nocardia;GORDON R. E.;J. Gen. Microbiol.,1962

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