Affiliation:
1. Department of Biological Chemistry, University of Maryland, School of Medicine, Baltimore, Maryland 21201
Abstract
Wild-type
Escherichia coli
B grew poorly on glutamate as the sole carbon source, except at very high concentrations of the amino acid. The addition of sodium ion markedly stimulated the growth. It had the same effect in a mutant of
E. coli
B selected for the ability to grow at low glutamate concentrations. Sodium ion also potentiated growth inhibition by analogues of glutamate. The uptake of glutamate by nongrowing cells of the mutant was markedly stimulated by sodium ion in the presence of an energy source, chloramphenicol, and arsenite, which retarded glutamate degradation.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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