Single-step PCR for detection of Brucella spp. from blood and milk of infected animals-Reference-Cited by-同舟云学术

Single-step PCR for detection of Brucella spp. from blood and milk of infected animals

Published:1995-12 Issue:12 Volume:33 Page:3087-3090
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Leal-Klevezas D S¹,Martínez-Vázquez I O¹,López-Merino A¹,Martínez-Soriano J P¹

Affiliation:

1. Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Monterrey, Nuevo León, Mexico.

Abstract

A versatile method for the extraction of Brucella DNA and PCR are presented as reliable tools for the detection of Brucella spp. from body fluids of infected animals. Two oligonucleotides homologous to regions of the gene encoding for an outer membrane protein (omp-2) were designed to detect the pathogen from milk and/or blood of infected goats, bovines, and human patients. The sensitivity of our test and its ability to detect the pathogen in samples from the field reveal a promising advance in the diagnosis of brucellosis in animals and humans.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.33.12.3087-3090.1995

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