Affiliation:
1. Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia, Brescia, Italy.
Abstract
The detection of foot-and-mouth disease virus (FMDV)-persistent carriers among convalescent ruminants is of paramount importance in the aftermath of a field outbreak. To this purpose, FMDV-specific antibody should be investigated first, since virus isolation procedures from such carriers are seriously constrained. The complexity of the overall picture may be compounded by possible emergency vaccinations in the affected areas at the beginning of the outbreak. In this case, it is suggested that mucosal rather than serum antibody be investigated. In fact, we showed that FMDV-infected cattle regularly mount an antibody response in oropharyngeal fluids, in contrast to vaccinated cattle. Antibody could be revealed by neutralization assays and/or an immunoglobulin A (IgA)-specific kinetic enzyme-linked immunosorbent assay (ELISA). Cattle vaccinated once seldom showed a mucosal antibody response, which could be only detected by a total immunoglobulin-specific kinetic ELISA. Very few, if any, cattle showed a mucosal IgA response after repeated vaccinations. Our kinetic, IgA-specific ELISA generally allowed an early detection of FMDV-infected cattle; in particular, it proved to be more sensitive than the usual indirect, antigen-trapping ELISA in experiments on saliva samples.
Publisher
American Society for Microbiology
Reference21 articles.
1. Ahl R. R. J. Lorenz and G. Wittman. 1988. Assessment of potency of foot-and-mouth disease vaccines by means of antibody assays with sera from vaccinated cattle p. 99-111. In Session of the Research Group of the European Commission for the Control of Foot-and-Mouth-Disease Prague Czechoslovakia 20-23 September. Food and Agriculture Organization Rome.
2. Isolation of mononuclear cytotoxic cells from cattle vaccinated against foot-and-mouth disease;Amadori M.;Arch. Virol.,1992
3. Amadori M. C. Berneri and E. Lodetti. 1992. Preparation storage and potency testing of concentrated inactivated FMDV antigens p. 20-22. In Session of the Research Group of the European Commission for the Control of Foot-and-Mouth Disease Mittelhäusern Switzerland 8-11 September. Food and Agriculture Organization Rome.
4. Influence of intranasal immunization with synthetic peptides corresponding to conserved epitopes of M protein on mucosal colonization by group A streptococci;Bessen D.;Infect. Immun.,1988
5. Transfer of functional immunoglobulin G (IgG) antibody into the gastrointestinal tract accounts for IgG clearance in calves;Besser T. E.;J. Virol.,1988
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