A New Highly Sensitive and Specific Real-Time PCR Assay Targeting the Malate Dehydrogenase Gene of Kingella kingae and Application to 201 Pediatric Clinical Specimens-Reference-Cited by-同舟云学术

A New Highly Sensitive and Specific Real-Time PCR Assay Targeting the Malate Dehydrogenase Gene of Kingella kingae and Application to 201 Pediatric Clinical Specimens

Published:2018-08 Issue:8 Volume:56 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

El Houmami Nawal¹^ORCID,Durand Guillaume André¹,Bzdrenga Janek²,Darmon Anne¹,Minodier Philippe³,Seligmann Hervé¹,Raoult Didier¹,Fournier Pierre-Edouard¹

Affiliation:

1. UMR VITROME, Aix-Marseille Université, IRD, Service de Santé des Armées, Assistance Publique-Hôpitaux de Marseille, Institut Hospitalo-Universitaire Méditerranée-Infection, Marseille, France

2. Université Grenoble Alpes, CEA, CNRS, IBS, Grenoble, France

3. Department of Pediatric Emergency Medicine, North Hospital, Marseille, France

Abstract

Kingella kingae is a significant pediatric pathogen responsible for bone and joint infections, occult bacteremia, and endocarditis in early childhood. Past efforts to detect this bacterium using culture and broad-range 16S rRNA gene PCR assays from clinical specimens have proven unsatisfactory; therefore, by the late 2000s, these were gradually phased out to explore the benefits of specific real-time PCR tests targeting the groEL gene and the RTX locus of K. kingae .

Funder

French National Research Agency

Foundation Meditérranée Infection

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00505-18

Reference41 articles.

1. Kingella kingae: Carriage, Transmission, and Disease

2. Outbreaks of Invasive Kingella kingae Infections in Daycare Facilities: Approach to Investigation and Management