Comparison of animal infectivity and excystation as measures of Giardia muris cyst inactivation by chlorine

Author:

Hoff J C,Rice E W,Schaefer F W

Abstract

In this study, in vitro excystation and mouse infectivity were compared as methods for quantitatively determining the viability of Giardia muris cysts before and after exposure to free residual chlorine. The mouse infectivity results show that very few cysts (1 to 15) constitute an infectious dose. The results of the inactivation studies indicate that in vitro excystation is an adequate indication of G. muris cyst infectivity for the host and can be used to determine the effects of disinfectants on cyst viability.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference18 articles.

1. American Public Health Association. 1980. Standard methods for the examination of water and wastewater 15th ed. American Public Health Association Washington D.C.

2. Giardia excystation can be induced in vitro in acidic solutions;Bingham A. K.;Nature (London),1979

3. Craun G. F. 1984. Waterborne outbreaks of giardiasis: current status p. 243-261. In D. L. Erlandsen and E. A. Meyer (ed.) Giardia and giradiasis. Plenum Press New York.

4. Finny D. J. 1978. Statistical method in biological assay 3rd ed p. 394-401. MacMillan Publishing Co. Inc. New York.

5. Effect of chlorine on Giardia lamblia cyst viability;Jarroll E.;Appl. Environ. Microbiol.,1981

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