Serodiagnosis of ram epididymitis by counterimmunoelectrophoresis, using Brucella ovis surface R antigen

Author:

Myers D M,Varela-Diaz V M

Abstract

The counterimmunoelectrophoresis (CIE) technique was developed as a diagnostic procedure for ram epididymitis caused by Brucella ovis. CIE test results with sera from naturally and experimentally infected male sheep compared favorably with those obtained by gel diffusion and complement fixation employing the same B. ovis surface R antigen. The main advantage of CIE over gel diffusion consists in a significant reduction of the time required to detect precipitin formation, whereas both methods obviate several of the difficulties encountered with complement fixation tests for B. ovis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference11 articles.

1. Epididymitis in rams. Studies on laboratory diagnosis;Biberstein E. L.;Cornell Vet.,1958

2. Vaccination in the control of Brucella ovis infection in sheep;Buddle M. B.;N. Z. Vet. J.,1958

3. A complement-fixation test for the diagnosis of ovine brucellosis with special reference to epididymitis;Clapp K. H.;Aust. Vet. J.,1955

4. A comparison of various antigens used in the complement-fixation test for ovine brucellosis;Clapp K. H.;Aust. Vet. J.,1961

5. Identification d'un compose antigenique specifique de la phase rugueuse (R) des;Diaz R.;Brucella. Ann. Rech. Vet.,1973

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