Identification of Staphylococcus aureus and Determination of Methicillin Resistance Directly from Positive Blood Cultures by Isothermal Amplification and a Disposable Detection Device-Reference-Cited by-同舟云学术

Identification of Staphylococcus aureus and Determination of Methicillin Resistance Directly from Positive Blood Cultures by Isothermal Amplification and a Disposable Detection Device

Published:2008-04 Issue:4 Volume:46 Page:1534-1536
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Goldmeyer James¹,Li Haijing²,McCormac Melinda³,Cook Steve⁴,Stratton Charles²³,Lemieux Bertrand¹,Kong Huimin¹,Tang Wen¹,Tang Yi-Wei²³

Affiliation:

1. BioHelix Corporation, Beverly, Massachusetts 01915

2. Departments of Medicine

3. Pathology, Vanderbilt University Medical Center, Nashville, Tennessee 37232

4. Lahey Clinic Medical Center, Burlington, Massachusetts 01805

Abstract

ABSTRACT A simple, rapid, and user-friendly procedure has been developed to identify Staphylococcus aureus and determine its methicillin resistance directly from gram-positive cocci in cluster-containing blood culture medium. The specimens were diluted and heated prior to amplification of the nuc and mecA genes with isothermal helicase-dependent amplification. Amplicons were detected using a disposable detection device. The analytical sensitivity of the assays was 50 CFU per reaction, and the clinical sensitivity and specificity were both 100% for S. aureus detection and 100% and 98% for methicillin resistance determination, respectively.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02234-07

Reference14 articles.

1. An, L., W. Tang, T. A. Ranalli, H. J. Kim, J. Wytiaz, and H. Kong. 2005. Characterization of a thermostable UvrD helicase and its participation in helicase-dependent amplification. J. Biol. Chem.280:28952-28958.

2. Bates, D. W., L. Goldman, and T. H. Lee. 1991. Contaminant blood cultures and resource utilization. The true consequences of false-positive results. JAMA265:365-369.

3. Carroll, K. C., R. B. Leonard, P. L. Newcomb-Gayman, and D. R. Hillyard. 1996. Rapid detection of the staphylococcal mecA gene from BACTEC blood culture bottles by the polymerase chain reaction. Am. J. Clin. Pathol.106:600-605.

4. Evaluation of Three Rapid Methods for the Direct Identification of Staphylococcus aureus from Positive Blood Cultures

5. Methicillin-Resistant Staphylococcus aureus: An Evolutionary, Epidemiologic, and Therapeutic Odyssey

Cited by 63 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Recent advances and challenges of biosensing in point-of-care molecular diagnosis;Sensors and Actuators B: Chemical;2021-12

2. Isothermal Amplification of Nucleic Acids: The Race for the Next “Gold Standard”;Frontiers in Sensors;2021-09-28

3. Developments in integrating nucleic acid isothermal amplification and detection systems for point-of-care diagnostics;Biosensors and Bioelectronics;2020-12

4. Rapid Detection of Methicillin-Resistant Staphylococcus aureus Directly from Blood for the Diagnosis of Bloodstream Infections: A Mini-Review;Diagnostics;2020-10-15

5. A fully integrated bacterial pathogen detection system based on count-on-a-cartridge platform for rapid, ultrasensitive, highly accurate and culture-free assay;Biosensors and Bioelectronics;2020-03