Birnavirus precursor polyprotein is processed in Escherichia coli by its own virus-encoded polypeptide

Author:

Jagadish M N1,Staton V J1,Hudson P J1,Azad A A1

Affiliation:

1. CSIRO, Division of Protein Chemistry, Parkville, Australia.

Abstract

The cDNA fragment of the large RNA segment of infectious bursal disease virus 002-73, when expressed in Escherichia coli, produces precursor polyprotein (N-VP2-VP4-VP3-C), most of which is then processed to generate constituent polypeptides. Using cDNA fragments containing site-specific mutations and two monoclonal antibodies that are specific to VP2 and VP3 of mature virus particles, we demonstrated that the VP4 protein is involved in processing of the precursor polyprotein to generate VP2 and VP3 and excluded the possibility of internal initiation for the generation of VP3.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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