Affiliation:
1. Department of Laboratory Medicine, University of Minnesota, Minneapolis, Minnesota 55455
Abstract
Precoated cellulose thin-layer chromatograms were used to detect the production of guanosine 5′-phosphate from guanosine and
p
-nitrophenylphosphate by whole-cell preparations. One-hundred per cent of
Serratia
(163 strains) and 84% of
E. liquefaciens
(15 of 18 strains) produced the nucleotide. All other
Enterobacter
(23 strains),
Klebsiella
(10 strains), and
E. coli
(10 strains) were negative for the production of this nucleotide. The entire test procedure could be carried out in 4 hr. It is proposed that
E. liquefaciens
is more closely related to
Serratia
than
Enterobacter
and that reclassification of these organisms should be investigated.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Cited by
6 articles.
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