Stability of Amphotericin B in Fungal Culture Media

Author:

Cheung S. C.12,Medoff G.12,Schlessinger D.12,Kobayashi G. S.12

Affiliation:

1. Infectious Disease and Dermatology Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

2. Department of Microbiology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110

Abstract

We have found that amphotericin B is unstable in two commonly employed fungal culture media. This instability leads to inaccuracies in determining the actual level of susceptibility of slow-growing strains that require prolonged incubation for growth. To help compensate for this problem, we have described two rapid methods of susceptibility testing.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference17 articles.

1. Rapid radiometric method of testing susceptibility of mycobacteria and slow-growing fungi to antimicrobial agents;Benitez P.;Antimicrob. Agents Chemother.,1974

2. Bennett J. E. 1967. Susceptibility of Cryptococcus neoforms to amphotericin B p. 405-410. Antimicrob. Agents Chemother. 1966.

3. Stability of amphotericin B in infusion bottles;Block E. R.;Antimicrob. Agents Chemother.,1973

4. In vitro susceptibility of isolates of Aspergillus fumigatus and Sporothrix schenckii to amphotericin B;Brandsberg J. W.;Antimicrob. Agents Chemother.,1972

5. Amphotericin B serum concentrations during therapy;Field B. T.;Appl. Microbiol.,1970

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