Affiliation:
1. RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
2. Science of Biological Supermolecular Systems, Graduate School of Integrated Science, Yokohama City University, Suehiro, Tsurumi-ku, Yokohama 230-0045, Japan
Abstract
ABSTRACT
Comamonas testosteroni
TA441 utilizes testosterone via aromatization of the A ring followed by
meta
-cleavage of the ring. The product of the
meta
-cleavage reaction, 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-dien-4-oic acid, is degraded by a hydrolase, TesD. We directly isolated and identified two products of TesD as 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid and (2
Z
,4
Z
)-2-hydroxyhexa-2,4-dienoic acid. The latter was a pure 4
Z
isomer. 2-Hydroxyhexa-2,4-dienoic acid was converted by a hydratase, TesE, and the product isolated from the reaction solution was identified as 2-hydroxy-4-hex-2-enolactone, indicating the direct product of TesE to be 4-hydroxy-2-oxohexanoic acid.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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