Affiliation:
1. Department of Medicine, Hospital General Universitari Vall d'Hebron, Barcelona, Spain.
Abstract
We have studied the distribution of viral sequences from the 5' noncoding region and from a fragment of the E2/NS2 region of the hepatitis C virus (HCV) genome in samples obtained before and after liver transplantation in two patients with HCV cirrhosis. The population of viral sequences in both regions were established by sequencing cloned PCR products. In both cases, the complexity of the viral quasispecies decreased after transplantation, although the consensus nucleotide and amino acid sequences remained unchanged. It is suggested that both positive and negative selection and random sampling events contribute substantially in shaping the genetic composition of HCV quasispecies and that recurrence of HCV infection may occur under equilibrium conditions.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference22 articles.
1. Detection of antibodies to hepatitis C virus in prospectively followed transfusion recipients with acute and chronic non-A, non-B hepatitis;Alter H.;N. Engl. J. Med.,1989
2. Alter M. J. 1991. Epidemiology of community-acquired hepatitis C p. 410-413. In F. B. Hollinger S. M. Lemon and H. Margolis (ed.) Viral hepatitis and liver disease. Williams and Wilkins Baltimore.
3. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction;Chomczynski P.;Anal. Biochem.,1987
4. Genetic organization and diversity of the hepatitis C virus;Choo Q.;Proc. Natl. Acad. Sci. USA,1991
5. Domingo E. and J. J. Holland. 1994. Mutation rates and rapid evolution of RNA viruses p. 161-184. In S. S. Morse (ed.) Evolutionary biology of viruses. Raven Press New York.
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