Affiliation:
1. Population Council, New York, New York 10021.
Abstract
We previously observed that when human immunodeficiency virus (HIV)-infected T lymphocytes are added to epithelial cells, they adhere, polarize, and secrete virions unidirectionally onto the epithelium. Epithelial cells subsequently take up virus and become productively infected. We report here that colchicine treatment of T-lymphocyte suspensions induced lymphocyte polarization, redistribution of F-actin into a pseudopod, and secretion of HIV from the pseudopod. Immobilization of T lymphocytes on negatively charged plastic also caused redistribution of F-actin and unidirectional secretion of HIV onto the plastic. As neither colchicine nor adhesion caused an increase in HIV secretion, they apparently act by focusing secretion to the tip of the pseudopod. We speculate that adhesion-induced polar secretion of HIV, from activated mononuclear cells onto epithelia, is a cytoskeleton-mediated process which may be involved in HIV transmission in vivo.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference34 articles.
1. Effects of disease stage and zidovudine therapy on the detection of human immunodeficiency virus type I in semen;Anderson D. J.;JAMA,1992
2. Characterization of human immunodeficiency virus type I strains recovered from the bowel of infected individuals;Barnett S. W.;Virology,1991
3. Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS);Barre-Sinoussi F.;Science,1983
4. Transmission of human immunodeficiency virus from monocytes to epithelia;Bourinbaiar A. S.;J. Acquired Immune Defic. Syndr.,1991
5. Polarization of NK cell cytoskeleton upon conjugation with sensitive target cells;Carpen O.;J. Immunol.,1983
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