Affiliation:
1. Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104
Abstract
The biological activities of
Bacillus subtilis
ribosomes pretreated with 0.5, 1, or 2 m
m
p
-chloromercuribenzoate (PCMB) were examined. The ribosomes treated with 1 or 2 m
m
PCMB lost their capacity to synthesize polyphenylalanine as well as the capacity to bind phenylalanyl-transfer ribonucleic acid. On the other hand, approximately 70% of the polyphenylalanine synthesis capacity was lost with ribosomes pretreated with 0.5 m
m
PCMB. This inhibition was found to be due to a specific inhibition of the translocation step. The effect of 0.5 m
m
PCMB was reversed by 20 m
m
Mg
2+
without the loss of PCMB bound to ribosomes, while β-mercaptoethanol partially reversed the inhibitory effect with concomitant loss of bound PCMB.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference24 articles.
1. A ribosomes-bound intermediate in polypeptide synthesis;Arlinghaus R.;Biochem. Biophys. Res. Commun.,1963
2. Bollum F. J. 1966. Filter paper disc techniques for assaying radioactive macromolecules p. 296-300. In G. L Cantoni and D. R. Davies (ed.) Procedures in nucleic acid research vol. 1. Harper and Row Publishers New York.
3. Boyer P. D. 1959. Sulfhydryl and disulfide groups of enzymes p. 511-588. In P. D. Boyer H. Lardy and K. Myrback (ed.) The enzymes vol. 1. Academic Press Inc. New York.
4. A simple efficient liquid scintillator for counting aqueous solution in a liquid scintillation counter;Bray G. A.;Anal. Biochem.,1960
5. The behaviour of acetylphenylalanyl soluble ribonucleic acid in polyphenylalanine synthesis;Haenni A. L.;Biochim. Biophys. Acta,1966
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