Few Highly Abundant Operational Taxonomic Units Dominate within Rumen Methanogenic Archaeal Species in New Zealand Sheep and Cattle

Abstract

ABSTRACTSequencing and analyses of 16S rRNA gene amplicons were performed to estimate the composition of the rumen methanogen community in 252 samples from eight cohorts of sheep and cattle, separated into 16 different sample groups by diet, and to determine which methanogens are most prominent in the rumens of farmed New Zealand ruminants.Methanobacteriales(relative abundance ± standard deviation, 89.6% ± 9.8%) andMethanomassiliicoccales(10.4% ± 9.8%) were the two major orders and contributed 99.98% (±0.1%) to the rumen methanogen communities in the samples. Sequences fromMethanobacterialeswere almost entirely from only four different species (or clades of very closely related species). Each was detectable in at least 89% of the samples. These four species or clades were theMethanobrevibacter gottschalkiiclade andMethanobrevibacter ruminantiumclade with a mean abundance of 42.4% (±19.5% standard deviation) and 32.9% (±18.8%), respectively, andMethanosphaerasp. ISO3-F5 (8.2% ± 6.7%) andMethanosphaerasp. group5 (5.6% ± 5.7%). These four species or clades appeared to be primarily represented by only one or, in one case, two dominant sequence types per species or clade when the sequences were grouped into operational taxonomic units (OTUs) at 99% sequence identity. The mean relative abundance ofMethanomassiliicoccalesin the samples was relatively low but exceeded 40% in some of the treatment groups. Animal feed affected the apparent methanogen community structure of both orders, as evident from differences in relative abundances of the major OTUs in animals under different feeding regimens.