Molecular typing of nosocomial strains of Legionella pneumophila by arbitrarily primed PCR-Reference-Cited by-同舟云学术

Molecular typing of nosocomial strains of Legionella pneumophila by arbitrarily primed PCR

Published:1996-06 Issue:6 Volume:34 Page:1595-1598
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Grattard F¹,Berthelot P¹,Reyrolle M¹,Ros A¹,Etienne J¹,Pozzetto B¹

Affiliation:

1. Laboratoire de Bactériologie-Virologie, Faculté de Médecine J. Lisfranc, Saint-Etienne, France.

Abstract

Arbitrarily primed PCR with two different primers was compared with ribotyping and monoclonal antibody analysis for typing Legionella strains. Applied to 11 epidemiologically unrelated strains, arbitrarily primed PCR resulted in an index of discrimination of 100% with both primers. It was found able to identify an epidemic clone of Legionella pneumophila serogroup 1 that was isolated from both patients and a hot water circuit of the same hospital.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.34.6.1595-1598.1996

Reference16 articles.

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2. Molecular fingerprinting of Legionella species by repetitive element PCR;Georghiou P. R.;J. Clin. Microbiol.,1994

3. Comparison of arbitrarily primed polymerase chain reaction, ribotyping and monoclonal antibody analysis for subtyping Legionella pneumophila serogroup 1;Gomez-Lus P.;J. Clin. Microbiol.,1993

4. Arbitrarily primed PCR, ribotyping, and plasmid pattern analysis applied to investigation of a nosocomial outbreak due to Enterobacter cloacae in a neonatal intensive care unit;Grattard F.;J. Clin. Microbiol.,1994

5. Differentiation of Pseudomonas aeruginosa strains by ribotyping: high discriminatory power by using a single restriction endonuclease;Grattard F.;J. Med. Microbiol.,1994

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