Highly Sensitive Lineage Discrimination of SARS-CoV-2 Variants through Allele-Specific Probe PCR-Reference-Cited by-同舟云学术

Highly Sensitive Lineage Discrimination of SARS-CoV-2 Variants through Allele-Specific Probe PCR

Published:2022-04-20 Issue:4 Volume:60 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Ratcliff Jeremy¹^ORCID,Al-Beidh Farah²³,Bibi Sagida⁴,Bonsall David⁵^ORCID,Costa Clemens Sue Ann⁴⁶,Estcourt Lise⁷⁸,Evans Amy⁸,Fish Matthew⁹¹⁰¹¹,Folegatti Pedro M.¹²,Gordon Anthony C.²³,Jay Cecilia¹^ORCID,Jennings Aislinn⁹¹⁰¹¹,Laing Emma⁸,Lambe Teresa¹²,MacIntyre-Cockett George⁵,Menon David¹³,Mouncey Paul R.¹⁴,Nguyen Dung¹,Pollard Andrew J.⁴¹⁵,Ramasamy Maheshi N.⁴¹⁶,Roberts David J.⁷¹⁷,Rowan Kathryn M.¹⁴,Rynne Jennifer⁹¹⁰¹¹,Shankar-Hari Manu⁹¹⁰¹¹^ORCID,Williams Sarah¹¹⁶,Harvala Heli¹⁸,Golubchik Tanya⁵^ORCID,Simmonds Peter¹^ORCID

Affiliation:

1. Peter Medawar Building for Pathogen Research, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom

2. Imperial College London, London, United Kingdom

3. Imperial College Health NHS Trust, St. Mary’s Hospital, London, United Kingdom

4. Oxford Vaccine Group, Department of Paediatrics, University of Oxford, Oxford, United Kingdom

5. Big Data Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom

6. Institute for Global Health, University of Siena, Siena, Italy

7. Haematology Theme, Radcliffe Department of Medicine, University of Oxford, Oxford, United Kingdom

8. Clinical Trials Unit, NHS Blood and Transport, Oxford, United Kingdom

9. School of Immunology and Microbial Sciences, Kings College London, London, United Kingdom

10. Guy’s and St. Thomas’ NHS Foundation Trust, St. Thomas’ Hospital, London, United Kingdom

11. Centre for Inflammation Research, The University of Edinburgh, Edinburgh, United Kingdom

12. Centre for Clinical Vaccinology and Tropical Medicine, The Jenner Institute, University of Oxford, Oxford, United Kingdom

13. University Division of Anaesthesia, University of Cambridge, Cambridge, United Kingdom

14. Intensive Care National Audit and Research Centre, London, United Kingdom

15. NIHR Oxford Biomedical Research Centre, Oxford, United Kingdom

16. Oxford University Hospitals NHS Foundation Trust, Oxford, United Kingdom

17. Clinical, Research, and Development, NHS Blood and Transport, Oxford, United Kingdom

18. Microbiology Services, NHS Blood and Transport, London, United Kingdom

Abstract

Tools to detect SARS-CoV-2 variants of concern and track the ongoing evolution of the virus are necessary to support public health efforts and the design and evaluation of novel COVID-19 therapeutics and vaccines. Although next-generation sequencing (NGS) has been adopted as the gold standard method for discriminating SARS-CoV-2 lineages, alternative methods may be required when processing samples with low viral loads or low RNA quality.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.02283-21

Reference29 articles.

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