Abstract
A membrane filter procedure is described for the enumeration of Candida albicans in natural waters. Several hundred milliliters of sample can be examined by filtration through 1.2-micrometer membranes. Selectivity is achieved by the use of a defined (yeast-nitrogen base plus maltos-) agar medium inclusion of the antimicrobial agents chloramphenicol and cycloheximide, and incubation at 37 degrees C. C. albicans colonies are differentiated primarily through color by use of a bismuth salt indicator system. Average recovery of various strains of C. albicans stressed in seawater at 4 degrees C was 82%, compared with those of spread plate controls on a noninhibitory medium. With river water and raw sewage, 90% of typical C. albicans colonies were confirmed as such in a simplified germ tube test. Atypical colonies verified as C. albicans were infrequent (3%). C. tropicalis and Torulopsis candida were the most common false-positive colonies.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference28 articles.
1. Ahearn D. G. 1974. Identification and ecology of yeasts of medical importance p. 129-146. In J. E. Prier and H. Friedman (ed.) Opportunistic pathogens. University Park Press Baltimore.
2. Ecology and characterization of yeasts from aquatic regions of South Florida;Abearn D. G.;Mar. Biol.,1968
3. Aerobic microbial flora of intertrigenous skin;Aly R.;Appl. Environ. Microbiol.,1977
4. Chronic mucocutaneous candidosis: a review;Aronson I. K.;Mycopathologia,1976
5. Distribution of aquatic yeasts-effect of incubation temperature and chloramphenicol concentration on isolation;Buck J. D.;Mycopathologia,1975
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