Pooling of Nasopharyngeal Swab Samples To Overcome a Global Shortage of Real-Time Reverse Transcription-PCR COVID-19 Test Kits-Reference-Cited by-同舟云学术

Pooling of Nasopharyngeal Swab Samples To Overcome a Global Shortage of Real-Time Reverse Transcription-PCR COVID-19 Test Kits

Published:2021-03-19 Issue:4 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

More Sunil¹,Narayanan Sai¹,Patil Girish¹,Ghosh Parna¹,Pushparaj Samuel¹,Cooper Emily¹,Ritchey Jerry¹,Cheruvu Vinay K.²,Kaul Anil¹³,Ramachandran Akhilesh¹^ORCID

Affiliation:

1. Oklahoma Animal Disease Diagnostic Laboratory, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma, USA

2. College of Public Health, Kent State University, Kent, Ohio, USA

3. Oklahoma State University Center for Health Sciences, Tulsa, Oklahoma, USA

Abstract

The global outbreak and rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has created an urgent need for large-scale testing of populations. There is a demand for high-throughput testing protocols that can be used for efficient and rapid testing of clinical specimens. We evaluated a pooled PCR protocol for testing nasopharyngeal (NP) swabs using known positive/negative and untested clinical samples that were assigned to pools of 5 or 10.

Funder

Oklahoma State University

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01295-20

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