Quantitative Real-Time PCR Is Not More Sensitive than “Conventional” PCR

Author:

Bastien Patrick1,Procop Gary W.2,Reischl Udo3

Affiliation:

1. University of Montpellier 1/Centre Hospitalier Universitaire de Montpellier, Laboratory of Parasitology-Mycology, Pôle Biologie Moléculaire du Centre National de Référence de la Toxoplasmose, Faculty of Medicine, Montpellier, France

2. Molecular Microbiology, Mycology, and Parasitology, Cleveland Clinic, Pathology and Laboratory Medicine Institute/L11, Cleveland, Ohio

3. Institute of Medical Microbiology and Hygiene, University of Regensburg, Regensburg, Germany

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference40 articles.

1. In-House Nucleic Acid Amplification Assays in Research: How Much Quality Control Is Needed before One Can Rely upon the Results?

2. Bastien, P. 2002. Molecular diagnosis of toxoplasmosis. Trans. R. Soc. Trop. Med. Hyg.96(Suppl. 1):S205-S215.

3. Beld, M. G. H. M., C. J. Birch, P. Cane, J. P. Clewley, E. Domingo, C. Escarmis, R. A. M. Fouchier, L. Jennings, I. D. Kay, O. Landt, S. Palladino, N. G. Papadopoulos, A. Petrich, M. W. Pfaffl, W. Rawlinson, U. Reischl, N. Saunders, C. Savolainen-Kopra, T. P. Sloots, Y. W. Tang, and P. C. Y. Woo. 2007. Experts roundtable: real-time PCR and microbiology, p. 357-443. In I. M. Mackay (ed.), Real-time PCR in microbiology: from diagnosis to characterization. Caister Academic Press, Norwich, United Kingdom.

4. Comparison of PCR Assays for Diagnosis of Cutaneous Leishmaniasis

5. Multicenter Quality Control for the Detection of Hepatitis C Virus RNA in Seminal Plasma Specimens

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