Comparison of Test Specificities of Commercial Antigen-Based Assays and In-House PCR Methods for Detection of Rotavirus in Stool Specimens-Reference-Cited by-同舟云学术

Comparison of Test Specificities of Commercial Antigen-Based Assays and In-House PCR Methods for Detection of Rotavirus in Stool Specimens

Published:2014-10-22 Issue:1 Volume:53 Page:295-297
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J. Clin. Microbiol.

Author:

Ye S.,Lambert S. B.,Grimwood K.,Roczo-Farkas S.,Nimmo G. R.,Sloots T. P.,Kirkwood C. D.,Whiley D. M.

Abstract

Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one commercial ICT, all the assays were generally reliable for rotavirus detection.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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