Novel Double-Antigen Sandwich Immunoassay for Human Hepatitis B Core Antibody-Reference-Cited by-同舟云学术

Novel Double-Antigen Sandwich Immunoassay for Human Hepatitis B Core Antibody

Published:2010-03 Issue:3 Volume:17 Page:464-469
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

Li An¹²³,Yuan Quan¹²³,Huang Zhiyin¹²³,Fan Jian¹²³,Guo Ruyi¹²³,Lou Bin¹²³,Zheng Qin¹²³,Ge Shengxiang¹²³,Chen Yixin¹²³,Su Zhijun¹²³,Yeo Anthony E. T.¹²³,Chen Yu¹²³,Zhang Jun¹²³,Xia Ningshao¹²³

Affiliation:

1. National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Life Science, Xiamen University, Xiamen, China

2. First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China

3. Quanzhou First Hospital of Fujian Medical University, Quanzhou, China

Abstract

ABSTRACT A novel diagnostic immunoassay testing procedure for hepatitis B virus core antibody (anti-HBc) using homogeneous purified full-length hepatitis B virus core antigen (HBcAg) capsids obtained from Escherichia coli was compared with Abbott Architect anti-HBc chemiluminescent microparticle immunoassay (CMIA; indirect method) against a library of specimens. A monoclonal anti-HBc neutralization confirmatory assay was then used to determine the degree of discordance between specimens. The new assay was found to be superior in both sensitivity and specificity.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CVI.00457-09

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