Transcription of BK virus DNA by Escherichia coli RNA polymerase: size and sequence analysis of RNA

Abstract

Transcription of human papovarirus BK superhelical DNA by Escherichia coli RNA polymerase yielded symmetric RNA with an average chain length of 1,3000 nucleotides. All regions of human papovavirus BK DNA were equally transcribed. At least four initiation sites were available to the procaryotic enzyme.