Affiliation:
1. Department of Microbiology, University of Pittsburgh, School of Medicine, Pittsburgh, Pennsylvania 15213
Abstract
Cell-free extract (crude extract) of
Agrobacterium tumefaciens
grown on
d
-glucuronate or
d
-glucarate converts
d
-glucarate and galactarate to a mixture of 2-keto-3-deoxy- and 4-deoxy-5-keto-
d
-glucarate. These compounds are then converted by partially purified crude extract to an intermediate tentatively identified as 2,5-diketoadipate. The same enzyme preparation further decarboxylates this intermediate to α-ketoglutarate semialdehyde, which is subsequently oxidized in a nicotinamide adenine dinucleotide-dependent reaction to α-ketoglutaric acid. Since
A. tumefaciens
converts
d
-glucuronic acid to
d
-glucarate, a pathway from
d
-glucuronate to α-ketoglutarate in
A. tumefaciens
was determined.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
33 articles.
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