Enhancement of Glutamic-Pyruvic Transaminase Activity in Leptospirae by Addition of Rabbit Serum-Reference-Cited by-同舟云学术

Enhancement of Glutamic-Pyruvic Transaminase Activity in Leptospirae by Addition of Rabbit Serum

Published:1970-03 Issue:3 Volume:1 Page:215-218
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Mifuchi Ichiji¹,Hosoi Masaharu¹,Yamashita Tadashi¹

Affiliation:

1. Department of Microbiology, Shizuoka College of Pharmacy, Shizuoka, Japan

Abstract

Glutamic-pyruvic transaminase (GPT) in leptospirae was shown to have an optimal temperature of 30 C and p H 8.0. It was activated by cofactor pyridoxal phosphate, similar to GPT from other sources. However, GPT in all leptospirae, such as Leptospira canicola, L. icterohaemorrhagiae, L. autumnalis , and L. biflexa , evidently had less activity even when pyridoxal phosphate was present, in comparison with the cultivable spirochete Reiter treponeme and Streptococcus faecalis . The low level of activity of leptospiral GPT was enhanced more than 2.5-fold by the addition of about 10% rabbit serum, whereas GPT of S. faecalis did not show such enhancement. Enhancement of leptospiral GPT was greater with rabbit serum than with bovine or horse serum.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/iai.1.3.215-218.1970

Reference24 articles.

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