Multiplex Amplified Nominal Tandem-Repeat Analysis (MANTRA), a Rapid Method for Genotyping Mycobacterium tuberculosis by Use of Multiplex PCR and a Microfluidic Laboratory Chip-Reference-Cited by-同舟云学术

Multiplex Amplified Nominal Tandem-Repeat Analysis (MANTRA), a Rapid Method for Genotyping Mycobacterium tuberculosis by Use of Multiplex PCR and a Microfluidic Laboratory Chip

Published:2010-10 Issue:10 Volume:48 Page:3758-3761
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Merritt Adam J.¹,Keehner Terillee¹,O'Reilly Lyn C.¹,McInnes Russell L.²,Inglis Timothy J. J.¹³

Affiliation:

1. Division of Microbiology & Infectious Diseases, PathWest Laboratory Medicine WA, Nedlands, WA 6009, Australia

2. Agilent Technologies, Forest Hill, Victoria, Australia

3. Microbiology & Immunology, School of Biomedical, Biomolecular & Chemical Sciences, Faculty of Life & Applied Sciences, University of Western Australia, Perth, Australia

Abstract

ABSTRACT A variable-number tandem-repeat genotyping method for Mycobacterium tuberculosis was converted to run in a multiplex PCR format on a 12-well microfluidic laboratory chip. Epidemiologically and genotypically distinct isolate clusters of M. tuberculosis were identified. This rapid genotyping method has potential application in smaller clinical laboratories and public health field investigations.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00471-10

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