Affiliation:
1. Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, Italy. rmangane@bgsm.edu
Abstract
Conjugative transposons are genetic elements able to promote their own intracellular transposition and intercellular conjugal transfer. They move by an excision-integration system related to that of lambdoid phages, in which the first step is the excision of the transposon from the donor replicon to form a covalently closed circular intermediate which contains a heteroduplex joint. In this work, sequencing both strands of the circular intermediate heteroduplex joint, it was found that, as during lambda phage excision, Tn916 excises from the host DNA by 5'-protruding staggered endonucleolytic cleavages.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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