Chloramphenicol causes fusion of separated nucleoids in Escherichia coli K-12 cells and filaments-Reference-Cited by-同舟云学术

Chloramphenicol causes fusion of separated nucleoids in Escherichia coli K-12 cells and filaments

Published:1996-07 Issue:14 Volume:178 Page:4289-4293
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

van Helvoort J M¹,Kool J¹,Woldringh C L¹

Affiliation:

1. Section of Molecular Cytology, Institute for Molecular Cell Biology, BioCentrum, University of Amsterdam, The Netherlands.

Abstract

Chloramphenicol is frequently used for better visualization of the Escherichia coli nucleoid. Here, we show that chloramphenicol causes not only rounding off of the nucleoid but also fusion of as many as four separated nucleoids. Nucleoid fusion occurred in fast-growing cells and in filaments obtained by dicF antisense RNA induction or in ftsZ84(Ts) and pbpB(Ts) mutants. Thus, treatment with chloramphenicol erroneously suggests that DNA segregation is inhibited.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.178.14.4289-4293.1996

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