Affiliation:
1. Department of Biochemistry, The University of Texas (Southwestern) Medical School at Dallas, Dallas, Texas 75235
Abstract
The cytochrome and nonheme iron protein content of two pseudomonads,
Pseudomonas oleovorans
and
P. putida
, containing mixed function oxidase systems was examined. The mixed function oxidase system of
P. oleovorans
and
P. putida
had previously been shown to be present in cells which had been grown on hexane and camphor, respectively, as energy source. The content of protoheme was found to increase significantly when the organisms were grown on the substrates for mixed function oxidation. The nonheme iron protein content increased significantly in the case of
P. putida
and was constant in
P. oleovorans
. The cytochrome
c
content was essentially constant in both pseudomonads. The content of cytochrome P-450 in
P. putida
increased from an immeasurably low amount to 0.15 nmoles per mg (dry weight). The content of cytochrome
o
in
P. oleovorans
increased by a factor of 4.5.
P. oleovorans
was not found to contain detectable quantities of cytochrome P-450 either in the presence or absence of the mixed function oxidase system.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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