Affiliation:
1. Department of Bacteriology and Immunology, School of Medicine, University of North Carolina, Chapel Hill 27515
Abstract
A method is described for predetermining whether lysozyme-damaged enterococci grow as either the parent strain or as L-forms. Organisms treated with lysozyme grew as L-forms on media solidified with low concentrations of agar, or the damaged cells grew as streptococci on media solidified with high concentrations of gelatin. After induction, some of the L-forms reverted to the parent strain, but most did not during three routine subcultures. Continued spontaneous reversion occurred through approximately 30 subcultures after induction. However, subsequent progeny did not revert, even when subjected to conditions such as the gelatin medium which strongly favors growth in the streptococcal phase.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference11 articles.
1. Reversion of L forms and spheroplasts of Proteus mirabilis;Altenbern R. A.;J. Bacteriol.,1963
2. Isolation and reversion of [-forms of beta-haemolytic streptococci;Crawford Y. E.;J. Infec. Dis.,1958
3. The L forms of bacteria;Dienes L.;Bacteriol. Rev.,1951
4. Studies of L-forms and protoplasts of group A streptococci. 1. Isolation, growth, and bacteriologic characteristics;Freimer E. H.;J. Exp. Med.,1959
5. Gooder H. and W. R. Maxted. 1961. External factors influencing structure and activities of Streptococcus pyogelnes p. 151-173. In G. G. Meynell and H. Gooder (ed.) Microbial reaction to environment. Sym. Soc. Gen. Microbiol. vol. 11 Cambridge University Press Cambridge England.
Cited by
25 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献