Development of a Two-Color Fluorescence In Situ Hybridization Technique for Species-Level Identification of Human-Infectious Cryptosporidium spp-Reference-Cited by-同舟云学术

Development of a Two-Color Fluorescence In Situ Hybridization Technique for Species-Level Identification of Human-Infectious Cryptosporidium spp

Published:2009-09-15 Issue:18 Volume:75 Page:5996-5998
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Alagappan A.¹,Bergquist P. L.¹²,Ferrari B. C.³

Affiliation:

1. Department of Chemistry and Biomolecular Science and Environmental Biotechnology CRC, Macquarie University, Sydney, New South Wales, Australia

2. Department of Molecular Medicine and Pathology, University of Auckland Medical School, Auckland, New Zealand

3. School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia

Abstract

ABSTRACT A two-color fluorescence in situ hybridization assay that allows for the simultaneous identification of Cryptosporidium parvum and C. hominis was developed. The assay is a simple, rapid, and cost-effective tool for the detection of the major Cryptosporidium species of concern to public health.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00643-09

Reference24 articles.

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2. Amann, R., and B. Fuchs. 2008. Single-cell identification in microbial communities by improved fluorescence in situ hybridization techniques. Nat. Rev. Microbiol.6:339-348.

3. Phylogenetic identification and in situ detection of individual microbial cells without cultivation

4. Amann, R. I., B. J. Binder, R. J. Olson, S. W. Chisholm, R. Devereux, and D. A. Stahl. 1990. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol.56:1919-1925.

5. Angus, K. W. 1983. Cryptosporidiosis in man, domestic animals and birds: a review. J. R. Soc. Med.76:62-70.

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