Affiliation:
1. Technical Resources Inc. and U.S. Environmental Protection Agency Environmental Research Laboratory, 2 Gulf Breeze, Florida 32561
Abstract
o
-Cresol and 3-methylcatechol were identified as successive transitory intermediates of toluene catabolism by the trichloroethylene-degrading bacterium G4. The absence of a toluene dihydrodiol intermediate or toluene dioxygenase and toluene dihydrodiol dehydrogenase activities suggested that G4 catabolizes toluene by a unique pathway. Formation of a hybrid species of
18
O- and
16
O-labeled 3-methylcatechol from toluene in an atmosphere of
18
O
2
and
16
O
2
established that G4 catabolizes toluene by successive monooxygenations at the
ortho
and
meta
positions. Detection of trace amounts of 4-methylcatechol from toluene catabolism suggested that the initial hydroxylation of toluene was not exclusively at the
ortho
position. Further catabolism of 3-methylcatechol was found to proceed via catechol-2,3-dioxygenase and hydroxymuconic semialdehyde hydrolase activities.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference29 articles.
1. Isolation of a Pseudoinonas stitzeri strain that degrades o-xylene;Baggi G.;Appl. Environ. Microbiol.,1987
2. Metabolism of phenol and cresols by mutants of Pseuidom 0onas piitida;Bayly R. C.;J. Bacteriol.,1973
3. The bacterial degradation of catechol;Dagley S.;Biochem. J.,1965
4. Expression of naphthalene oxidation genes in Escherichia co/i results in the biosynthesis of indigo;Ensley B. D.;Science,1983
5. Gibson D. T. 1971. Assay of enzymes of aromatic metabolism p. 462-471. In J. R. Norris and D. W. Ribbons (ed.). Methods in microbiology vol. 6A. Academic Press. Inc.. New York.
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