Biodegradation of Asphalt Cement-20 by Aerobic Bacteria

Author:

Pendrys John P.1

Affiliation:

1. Code R301, Naval Surface Warfare Center, Silver Spring, Maryland 20903-5000

Abstract

Seven gram-negative, aerobic bacteria were isolated from a mixed culture enriched for asphalt-degrading bacteria. The predominant genera of these isolates were Pseudomonas, Acinetobacter, Alcaligenes, Flavimonas , and Flavobacterium . The mixed culture preferentially degraded the saturate and naphthene aromatic fractions of asphalt cement-20. A residue remained on the surface which was resistant to biodegradation and protected the underlying asphalt from biodegradation. The most potent asphalt-degrading bacterium, Acinetobacter calcoaceticus NAV2, excretes an emulsifier which is capable of emulsifying the saturate and naphthene aromatic fractions of asphalt cement-20. This emulsifier is not denatured by phenol.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference23 articles.

1. D 4124 standard test method for separation of asphalt into four fractions, p. 697-701. In R. A. Priemon-Storer (ed.), Road and paving materials; traveled surface characteristics;American Society for Testing and Materials.;Philadelphia,1985

2. Microbial degradation of petroleum hydrocarbons: an environmental perspective;Atlas R. M.;Microbiol. Rev.,1981

3. Surface-active compounds from microorganisms;Cooper D. G.;Adv. Appl. Microbiol.,1980

4. Hydrocarbon-degrading bacteria associated with arctic oil seeps;Cundell A. M.;Dev. Ind. Microbiol.,1974

5. Emulsan in Acinetobacter calcoaceticus RAG-1: distribution of cell-free and cell-associated cross-reacting material;Goldman S.;Appl. Environ. Microbiol.,1982

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