Antibodies to nystatin demonstrate polyene sterol specificity and allow immunolabeling of sterols in Saccharomyces cerevisiae

Author:

Walker-Caprioglio H M1,MacKenzie J M1,Parks L W1

Affiliation:

1. Department of Microbiology, North Carolina State University, Raleigh 27695-7615.

Abstract

Polyclonal antibodies elicited by injection into rabbits of a nystatin-bovine serum albumin conjugate were reactive with both nystatin and amphotericin B. Upon labeling of polyene-treated Saccharomyces cerevisiae sterol auxotrophs grown on various sterols, nystatin reacted specifically with ergosterol, while amphotericin B did not react preferentially with ergosterol, cholesterol, or cholestanol. Time course labeling experiments demonstrated the rate of ergosterol transport into cholesterol-grown cells.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference15 articles.

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4. Rates of amphotericin B and filipin association with sterols;Clejan S.;J. Biol. Chem.,1985

5. Studies on the nature of the primary lesion induced by nystatin in the cell-membranes of Saccharomyces cerevisiae;Eilam Y.;Microbios Lett.,1982

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