Comparative Investigation into Formycin A and Pyrazofurin A Biosynthesis Reveals Branch Pathways for the Construction of C -Nucleoside Scaffolds-Reference-Cited by-同舟云学术

Comparative Investigation into Formycin A and Pyrazofurin A Biosynthesis Reveals Branch Pathways for the Construction of C -Nucleoside Scaffolds

Published:2020-01-07 Issue:2 Volume:86 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Zhang Meng¹,Zhang Peichao¹²,Xu Gudan¹,Zhou Wenting¹,Gao Yaojie¹,Gong Rong¹,Cai You-Sheng¹,Cong Hengjiang³,Deng Zixin¹,Price Neil P. J.⁴,Mao Xiangzhao²,Chen Wenqing¹^ORCID

Affiliation:

1. Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan, China

2. College of Food Science and Engineering, Ocean University of China, Qingdao, China

3. College of Chemistry and Molecular Sciences, Institute for Advanced Studies, Wuhan University, Wuhan, China

4. Agricultural Research Service, U.S. Department of Agriculture, National Center for Agricultural Utilization Research, Peoria, Illinois, USA

Abstract

FOR-A and PRF-A are C -nucleoside antibiotics known for their unusual chemical structures and remarkable biological activities. Deciphering the enzymatic mechanism for the construction of a C -nucleoside scaffold during FOR-A/PRF-A biosynthesis will not only expand the biochemical repertoire for novel enzymatic reactions but also permit target-oriented genome mining of FOR-A/PRF-A-related C -nucleoside antibiotics. Moreover, the availability of FOR-A/PRF-A biosynthetic gene clusters will pave the way for the rational generation of designer FOR-A/PRF-A derivatives with enhanced/selective bioactivity via synthetic biology strategies.

Funder

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01971-19

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