Advancing Desulfurization in the Model Biocatalyst <i>Rhodococcus qingshengii</i> IGTS8 via an <i>In Locus</i> Combinatorial Approach-Reference-Cited by-同舟云学术

Advancing Desulfurization in the Model Biocatalyst Rhodococcus qingshengii IGTS8 via an In Locus Combinatorial Approach

Published:2023-02-28 Issue:2 Volume:89 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Martzoukou Olga¹^ORCID,Amillis Sotiris¹,Glekas Panayiotis D.¹,Breyanni Dimitra²,Avgeris Margaritis³⁴,Scorilas Andreas³,Kekos Dimitris²,Pachnos Michalis⁵,Mavridis George⁵,Mamma Diomi²,Hatzinikolaou Dimitris G.¹^ORCID

Affiliation:

1. Enzyme and Microbial Biotechnology Unit, Department of Biology, National and Kapodistrian University of Athens, Attica, Greece

2. Biotechnology Laboratory, Sector of Synthesis and Development of Industrial Processes (IV), School of Chemical Engineering, National Technical University of Athens, Athens, Greece

3. Sector of Biochemistry and Molecular Biology, Department of Biology, National and Kapodistrian University of Athens, Attica, Greece

4. Laboratory of Clinical Biochemistry–Molecular Diagnostics, Second Department of Pediatrics, School of Medicine, National and Kapodistrian University of Athens, P. & A. Kyriakou Children’s Hospital, Athens, Greece

5. Division of European Affairs, Motor Oil Hellas, Marousi, Athens, Greece

Abstract

Rhodococcus is perhaps the most promising biodesulfurization genus and is able to withstand the harsh process conditions of a biphasic biodesulfurization process. In the present work, we constructed an advanced biocatalyst harboring a combination of three genetic modifications, namely, an operon rearrangement, a promoter exchange, and a gene overlap removal.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.01970-22

Reference63 articles.

1. Biodesulfurization: How to Make it Work?

2. Phylogenomic Classification and Biosynthetic Potential of the Fossil Fuel-Biodesulfurizing Rhodococcus Strain IGTS8

3. Identification and Cloning of Genes Involved in Specific Desulfurization of Dibenzothiophene by Rhodococcus sp. Strain IGTS8

4. Conservation of plasmid-encoded dibenzothiophene desulfurization genes in several rhodococci

5. Genomic structure and promoter analysis of the dsz operon for dibenzothiophene biodesulfurization from Gordonia alkanivorans RIPI90A

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