Author:
Thomsen Isaac P.,DuMont Ashley L.,James David B. A,Yoong Pauline,Saville Benjamin R.,Soper Nicole,Torres Victor J.,Creech C. Buddy
Abstract
ABSTRACTDespite the importance ofStaphylococcus aureusas a common invasive bacterial pathogen, the humoral response to infection remains inadequately defined, particularly in children. The purpose of this study was to assess the humoral response to extracellular staphylococcal virulence factors, including the bicomponent leukotoxins, which are critical for the cytotoxicity ofS. aureustoward human neutrophils. Children with culture-provenS. aureusinfection were prospectively enrolled and stratified by disease type. Fifty-three children were enrolled in the study, of which 90% had invasive disease. Serum samples were obtained during the acute (within 48 h) and convalescent (4 to 6 weeks postinfection) phases, at which point both IgG titers againstS. aureusexotoxins were determined, and the functionality of the generated antibodies was evaluated. Molecular characterization of clinical isolates was also performed. We observed a marked rise in antibody titer from acute-phase to convalescent-phase sera for LukAB, the most recently describedS. aureusbicomponent leukotoxin. LukAB production by the isolates was strongly correlated with cytotoxicityin vitro, and sera containing anti-LukAB antibodies potently neutralized cytotoxicity. Antibodies toS. aureusantigens were detectable in healthy pediatric controls but at much lower titers than in sera from infected subjects. The discovery of a high-titer, neutralizing antibody response to LukAB during invasive infections suggests that this toxin is producedin vivoand that it elicits a functional humoral response.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference43 articles.
1. National burden of invasive methicillin-resistant Staphylococcus aureus infections, United States, 2011;Dantes;JAMA Intern. Med.,2013
2. Staphylococcus aureus: superbug, super genome?;Lindsay;Trends Microbiol.,2004
3. The many faces of Staphylococcus aureus infection. Recognizing and managing its life-threatening manifestations;Weems;Postgrad. Med.,2001
4. Trends in invasive methicillin-resistant Staphylococcus aureus infections;Iwamoto;Pediatrics,2013
5. Staphylococcus aureus hemolysins, bi-component leukocidins, and cytolytic peptides: a redundant arsenal of membrane-damaging virulence factors?;Vandenesch;Front. Cell. Infect. Microbiol.,2012
Cited by
51 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献