Affiliation:
1. Department of Genetics, University of Melbourne, Parkville 3010, Australia
Abstract
ABSTRACT
The catabolism of fatty acids is important in the lifestyle of many fungi, including plant and animal pathogens. This has been investigated in
Aspergillus nidulans
, which can grow on acetate and fatty acids as sources of carbon, resulting in the production of acetyl coenzyme A (CoA). Acetyl-CoA is metabolized via the glyoxalate bypass, located in peroxisomes, enabling gluconeogenesis. Acetate induction of enzymes specific for acetate utilization as well as glyoxalate bypass enzymes is via the Zn
2
-Cys
6
binuclear cluster activator FacB. However, enzymes of the glyoxalate bypass as well as fatty acid beta-oxidation and peroxisomal proteins are also inducible by fatty acids. We have isolated mutants that cannot grow on fatty acids. Two of the corresponding genes,
farA
and
farB
, encode two highly conserved families of related Zn
2
-Cys
6
binuclear proteins present in filamentous ascomycetes, including plant pathogens. A single ortholog is found in the yeasts
Candida albicans
,
Debaryomyces hansenii
, and
Yarrowia lipolytica
, but not in the
Ashbya
,
Kluyveromyces
,
Saccharomyces
lineage. Northern blot analysis has shown that deletion of the
farA
gene eliminates induction of a number of genes by both short- and long-chain fatty acids, while deletion of the
farB
gene eliminates short-chain induction. An identical core 6-bp in vitro binding site for each protein has been identified in genes encoding glyoxalate bypass, beta-oxidation, and peroxisomal functions. This sequence is overrepresented in the 5′ region of genes predicted to be fatty acid induced in other filamentous ascomycetes,
C. albicans
,
D. hansenii
, and
Y. lipolytica
, but not in the corresponding genes in
Saccharomyces cerevisiae.
Publisher
American Society for Microbiology
Subject
Molecular Biology,General Medicine,Microbiology
Cited by
108 articles.
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