Self-Formed Adaptor PCR: a Simple and Efficient Method for Chromosome Walking-Reference-Cited by-同舟云学术

Self-Formed Adaptor PCR: a Simple and Efficient Method for Chromosome Walking

Published:2007-08 Issue:15 Volume:73 Page:5048-5051
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Wang Shiming¹²,He Jian¹,Cui Zhongli¹,Li Shunpeng¹

Affiliation:

1. Key Laboratory of Microbiological Engineering of Agricultural Environment, Department of Microbiology, Nanjing Agricultural University, Nanjing, People's Republic of China

2. Department of Bioengineering, School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing, People's Republic of China

Abstract

ABSTRACT We developed a self-formed adaptor PCR (termed SEFA PCR) which can be used for chromosome walking. Most of the amplified flanking sequences were longer than 2.0 kb, and some were as long as 6.0 kb. SEFA PCR is simple and efficient and should have broad applications in the isolation of unknown sequences in complex genomes.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02973-06

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