Affiliation:
1. Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, South Korea
Abstract
ABSTRACT
It is important to rapidly and selectively detect and analyze pathogenic
Salmonella enterica
subsp.
enterica
in contaminated food to reduce the morbidity and mortality of
Salmonella
infection and to guarantee food safety. In the present work, we developed an oligonucleotide microarray containing duplicate specific capture probes based on the
carB
gene, which encodes the carbamoyl phosphate synthetase large subunit, as a competent biomarker evaluated by genetic analysis to selectively and efficiently detect and discriminate three
S. enterica
subsp.
enterica
serotypes: Choleraesuis, Enteritidis, and Typhimurium. Using the developed microarray system, three serotype targets were successfully analyzed in a range as low as 1.6 to 3.1 nM and were specifically discriminated from each other without nonspecific signals. In addition, the constructed microarray did not have cross-reactivity with other common pathogenic bacteria and even enabled the clear discrimination of the target
Salmonella
serotype from a bacterial mixture. Therefore, these results demonstrated that our novel
carB
-based oligonucleotide microarray can be used as an effective and specific detection system for
S. enterica
subsp.
enterica
serotypes.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
8 articles.
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