A 50-Kilodalton Cry2A Peptide Is Lethal to Bombyx mori and Lymantria dispar-Reference-Cited by-同舟云学术

A 50-Kilodalton Cry2A Peptide Is Lethal to Bombyx mori and Lymantria dispar

Published:2012-07 Issue:13 Volume:78 Page:4755-4757
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Ohsawa Masataka¹,Tanaka Miki¹,Moriyama Kenta¹,Shimazu Mitsuaki²,Asano Shin-ichiro³,Miyamoto Kazuhisa⁴,Haginoya Kohsuke¹,Mitsui Toshiaki¹,Kouya Tomoaki⁵,Taniguchi Masayuki⁵,Hori Hidetaka¹

Affiliation:

1. Laboratory of Applied Biochemistry, Graduate School of Science and Technology, Niigata University, Niigata, Japan

2. Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, Japan

3. Graduate School of Agriculture, Hokkaido University, Hokkaido, Japan

4. Insect Pathology Laboratory, Department of Insect Genetics and Evolution, National Institute of Agrobiological Sciences, Tsukuba, Japan

5. Laboratory of Chemistry and Chemical Engineering, Graduate School of Science and Technology, Niigata University, Niigata, Japan

Abstract

ABSTRACT The Cry2Aa3 gene was introduced into asporogenic Bacillus thuringiensis , and the synthesized protoxin killed Bombyx mori and Lymantria dispar larvae. Chymotrypsin hydrolyzed the linkages between 49Tyr/Val50 and 145Lys/Ser146 in the protoxin, and 50- and 58-kDa fragments were generated, respectively. Both peptides killed the larvae of both insects.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.07123-11

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