Cas12a/Guide RNA-Based Platform for Rapid and Accurate Identification of Major Mycobacterium Species-Reference-Cited by-同舟云学术

Cas12a/Guide RNA-Based Platform for Rapid and Accurate Identification of Major Mycobacterium Species

Published:2020-01-28 Issue:2 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Xiao Guohui¹²,He Xing¹,Zhang Su¹,Liu Yaya¹,Liang Zhihang³,Liu Houming¹,Zhang Juanjuan¹,Ou Min¹,Cai Shuhao¹,Lai Wenjie¹,Zhang Tianyu²^ORCID,Ren Lili⁴^ORCID,Zhang Guoliang¹³

Affiliation:

1. National Clinical Research Center for Infectious Diseases, Guangdong Key Laboratory of Emerging Infectious Diseases, Shenzhen Third People’s Hospital, Southern University of Science and Technology, Shenzhen, China

2. State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences (CAS), Guangzhou, China

3. School of Basic Medical Sciences, Guangdong Medical University, Dongguan, China

4. Institute of Pathogen Biology (IPB), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China

Abstract

Mycobacterium tuberculosis infection and nontuberculous mycobacteria (NTM) infections exhibit similar clinical symptoms; however, the therapies for these two types of infections are different. Therefore, the rapid and accurate identification of M. tuberculosis and NTM species is very important for the control of tuberculosis and NTM infections. In the present study, a Cas12a/guide RNA (gRNA)-based platform was developed to identify M. tuberculosis and most NTM species.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01368-19

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