Affiliation:
1. Department of Chemistry, Western Michigan University, Kalamazoo, Michigan
Abstract
Ribosomes were isolated from three mesophilic and three thermophilic strains of
Bacillus
. The ribosomes consisted of about 55% protein and 45% ribonucleic acid. Average ratios for the absorbance at 260/235 and 260/280 mμ were 1.77 and 1.92 for the mesophiles and 1.63 and 1.84 for the thermophiles. Ultracentrifugation revealed mainly components with sedimentation coefficients of about 30, 50, 70, 100, and 120
S
. All the preparations were shown to contain a ribonuclease which, in the presence of ethylenediaminetetraacetic acid, led to ribosome breakdown as measured by the increase in acid-soluble nucleotides. The stability of the ribosomes from the thermophiles was consistently greater than that of the ribosomes from the mesophiles. After 5 hr at 37 C, the breakdown was about 80% for the ribosomes from the mesophiles and 55 to 70% for those from the thermophiles. At 60 C, the ribosomes from the mesophiles were broken down slightly more and at a faster rate than those from the thermophiles. At temperatures above 60 C, the breakdown was again more pronounced for the ribosomes from the mesophiles.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference3 articles.
1. ANFINSEN C. B. AND F. H. WHITE. 1961. The ribonucleases: occurrence structure and properties p. 95-122. In P. D. Boyer H. Lardy and K. Myrback [ed.] The enzymes vol. 5. Academic Press Inc. New York.
2. Thermal denaturation and amino acid binding ability of soluble RNA;ARCA M., C.;Biochem. Biophys. Res. Commun.,1963
3. ARCA M. C. CALVORI L. FRONTALI AND G. TECCE. 1964. The enzymic synthesis of aminoacyl derivatives of soluble ribonucleic acid from
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