Affiliation:
1. Division of Enteric Pathogens, Central Public Health Laboratory, Colindale, London, United Kingdom.
Abstract
Sera from 13 patients with hemolytic uremic syndrome (HUS) and 8 healthy control subjects were examined for antibodies specific for bacterial antigens of Eschericia coli serotype O157:H7. Bacterial components, including outer membrane proteins (OMPs), lipopolysaccharide (LPS), and flagella, were reacted with sera by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by immunoblotting and by enzyme-linked immunosorbent assay. All 13 serum samples from HUS patients contained high-titered antibodies of the immunoglobulin M class against O157 LPS and some OMPs. These same sera reacted weakly with some of the major OMPs, but not the LPS, of non-O157 strains of E. coli. Sera from patients did not contain antibodies to non-O157 LPS or H7 flagella. The possibility of using E. coli serotype O157 LPS in an enzyme-linked immunosorbent assay for the routine diagnostic testing of sera from HUS patients for evidence of O157:H7 infection is discussed.
Publisher
American Society for Microbiology
Reference38 articles.
1. Colorimetric determination of the components of 3,4 dihydroxyphenylalanine-tyrosine mixtures;Arnow L. E.;J. Biol. Chem.,1937
2. Antigenic S-type lipopolysaccharide of Brucella abortus 1119-3;Caroff M.;Infect. Immun.,1984
3. Iron regulated outer membrane proteins of Escherichia coli: variations in the expression due to the chelator used to restrict the availability of iron;Chart H.;J. Gen. Microbiol.,1986
4. Antigenic homology of the ferric enterobactin receptor protein of Escherichia coli;Chart H.;J. Gen. Microbiol.,1985
5. The serological relationship between Brucella spp., Yersinia enterocolitica serotype IX and Salmonella serotypes of Kauffmann-White group N;Corbell M. J.;J. Hyg.,1975
Cited by
106 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献