Changes in the rate of chitin-plus-chitosan synthesis accompany morphogenesis of Mucor racemosus

Author:

Domek D B,Borgia P T

Abstract

The in vivo differential rates of chitin-plus-chitosan biosynthesis in Mucor racemosus were determined under a variety of conditions, leading to yeast cell or mycelial morphology. Chitin-chitosan was determined as hot NaOH-insoluble radioactivity derived from N-acetyl-D-[1-3H]glucosamine in the medium. Control experiments demonstrated that the labeled material possessed the properties of chitin-plus-chitosan. Our results indicate that Mucor yeasts have a relatively low differential rate of chitin-plus-chitosan synthesis and that mycelial cells have a threefold-elevated differential rate. Treatment of aerobic cells with exogenous N6, O2-dibutyryl cyclic adenosine 3',5'-monophosphate, an agent which induces yeast cell morphology, also results in a lowered rate of chitin-plus-chitosan synthesis. Control experiments eliminated the possibility that the observed rate changes were due to changes in endogenous pool size, uptake of exogenous N-acetyl-p-[1-3H]glucosamine, or alterations in growth rate. Therefore, the changes are seemingly linked to morphogenesis. These results strengthen the idea that cyclic adenosine 3',5'-monophosphate plays an important role in dimorphism in Mucor. In addition, pulse-chase experiments suggest that considerable modification of newly synthesized chitin plus chitosan in both yeast cells and mycelia occurs in vivo.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference27 articles.

1. A pathway of chitosan formation in Mucor rouxii: enzymatic deacetylation of chitin;Araki Y.;Biochem. Biophys. Res. Comm.,1974

2. Cell wall chemistry, morphogenesis and taxonomy of fungi. Annu;Bartnicki-Garcia S.;Rev. Microbiol.,1968

3. Fundamental aspects of hyphal morphogenesis;Bartnicki-Garcia S.;Symp. Soc. Gen. Microbiol.,1973

4. Isolation of chitosomes from taxonomically diverse fungi and synthesis of microfibrils in vitro;Bartnicki-Garcia S.;Exp. Mycol.,1978

5. Control of ,Bglucosidase synthesis in Mucor racemosus;Borgia P. T.;J. Bacteriol.,1977

Cited by 20 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Chitosan-based nanoscale and non-nanoscale delivery systems for anticancer drugs: A review;European Polymer Journal;2021-07

2. Mucor indicus: Biology and industrial application perspectives: A review;Biotechnology Advances;2013-07

3. Metabolic Engineering of the Morphology of Aspergillus;Advances in Biochemical Engineering/Biotechnology;2001

4. Gene expression inMucordimorphism;Canadian Journal of Botany;1995-12-31

5. Yeast/Mycelial Dimorphism;Growth, Differentiation and Sexuality;1994

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3