A new method for direct detection of Listeria monocytogenes from foods by PCR-Reference-Cited by-同舟云学术

A new method for direct detection of Listeria monocytogenes from foods by PCR

Published:1995-10 Issue:10 Volume:61 Page:3745-3747
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Makino S¹,Okada Y¹,Maruyama T¹

Affiliation:

1. Department of Veterinary Public Health, Institute of Public Health, Tokyo, Japan.

Abstract

The preparation of DNA by alcohol precipitation in the presence of Na1 was used to enable the direct detection of Listeria monocytogenes in the amount of 10(3) CFU/0.5 g of sample. This procedure produces PCR-quality DNA directly from foods, such as soft cheese and minced meat.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.61.10.3745-3747.1995

Reference8 articles.

1. Listeria monocytogenes, a food-borne pathogen;Farber J. M.;Microbiol. Rev.,1991

2. Detection of Listeria monocytogenes in cheese with the magnetic immuno-polymerase chain reaction assay;Fluit A. C.;Appl. Environ. Microbiol.,1993

3. Simple procedure of DNA isolation from human serum;Ishizawa M.;Nucleic Acids Res.,1991

4. Enhanced sensitivity in PCR detection of Listeria monocytogenes in soft cheese through use of an aqueous two-phase system as a sample preparation method;Lantz P.;Appl. Environ. Microbiol.,1994

5. Direct detection of Bacillus anthracis DNA in animals by polymerase chain reaction;Makino S.;J. Clin. Microbiol.,1993

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