Detection of viable Cryptosporidium parvum oocysts by PCR-Reference-Cited by-同舟云学术

Detection of viable Cryptosporidium parvum oocysts by PCR

Published:1995-12 Issue:12 Volume:61 Page:4514-4516
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Wagner-Wiening C¹,Kimmig P¹

Affiliation:

1. Landesgesundheitsamt Baden-W�rttemberg, Stuttgart, Germany.

Abstract

PCR was used to detect and specifically identify a gene fragment from Cryptosporidium parvum. An 873-bp region of a 2,359-bp DNA fragment encoding a repetitive oocyst protein of C. parvum was shown to be specifically amplified in C. parvum. An excystation protocol before DNA extraction allowed the differentiation between live and dead Cryptosporidium parvum oocysts.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.61.12.4514-4516.1995

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