Quantification of Isoniazid-Heteroresistant Mycobacterium tuberculosis Using Droplet Digital PCR-Reference-Cited by-同舟云学术

Quantification of Isoniazid-Heteroresistant Mycobacterium tuberculosis Using Droplet Digital PCR

Published:2023-06-20 Issue:6 Volume:61 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Zhang Siqi¹,Chen Xiaohong²,Lin Zhonghui²,Tan Yaoju³,Liang Bin¹,Pan Yuying¹,Huang Mingxiang²,Su Biyi³,Hu Xiaoman¹,Xu Ye¹,Li Qingge¹^ORCID

Affiliation:

1. Engineering Research Centre of Molecular Diagnostics of Ministry of Education, State Key Laboratory of Cellular Stress Biology, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University, Xiamen, China

2. The Pulmonary Hospital of Fuzhou in Fujian Province, Fuzhou, Fujian, China

3. Department of Clinical Laboratory, Guangzhou Chest Hospital, Guangzhou, China

Abstract

The quantitative detection of drug-resistance mutations in Mycobacterium tuberculosis (MTB) is critical for determining the drug resistance status of a sample. We developed a drop-off droplet digital PCR (ddPCR) assay targeting all major isoniazid (INH)-resistant mutations.

Funder

Xiamen Major Science and Technology Project

Basic Research Project of Shenzhen Basic Research Program

Xiamen Industry-university-Research Project Subsidy Project

Major Science and Technology Project of Inner Mongolia province

Fujian University Industry-University-Research Joint Innovation Project

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.01884-22